Ere transfected overnight in the absence of antibiotics, and medium was replaced the next morning with Opti-MEM? FBS. Transfected cells were harvested at 44 h and RNA extracted with Trizol (Life Technologies, Inc.). To analyze encapsidated RNA, cells were resuspended in 10 mM Tris (pH 7.5)?0 mM NaCl? mM CaCl2? Triton X-100?.5 sodium deoxycholate containing 1 g of micrococcal nuclease (S7 nuclea


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