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D whether IGFBP3 modulates GIST cell response to imatinib, we first sought to determine whatPage 2 of(page number not for citation purposes)Molecular Cancer 2009, 8:http://www.molecular-cancer.com/content/8/1/GIST882 KIT exon 13: K642EGIST-T1 KIT exon 11: V560delYADBImatinib IGFBP3 -tubulin24 hr48 hrEImatinib IGFBP3 -tubulin24 hr48 hr-+-+-+-+3 BP F IG rhCFigure 1 Imatinib-induced IGFBP3 expression
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Tamembrane regulatory domain. Imatinib treatment resulted in a loss of viability of more than 60 at a concentration of 0.5 M, and the observed IC50 was 0.05 M at 48 and 72 hours (Figure 1D). In contrast to GIST882 cells, GIST-T1 cells did not have detectable basal levels of IGFBP3, nor did imatinib treatment induce IGFBP3 expression (Figure 1E). This data was a preliminary indicator that IGFBP3 i
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